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The first such method was developed in biophysicist Steve Quake’s lab at Caltech and commercialized by Helicos Biosciences, but became unavailable after the company declared bankruptcy, saddled by legal issues and unable to find a market niche. These days, the canonical technique comes from a company called Pacific Biosciences (PacBio). Scientists often refer to single-molecule techniques as “third-generation” DNA sequencing, though they’re often also lumped into the NGS bucket with Illumina.
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Those first five streams were "rough," Luke remembered. First he couldn't figure out how to get his full TikTok streams on YouTube. Then his internet started getting unusably laggy. (He wound up running a giant ethernet cable out to his "barn," the shed-studio that houses his streaming setup and DIY voice booth.) But he stuck with it. Eventually, Luke said, "I learned stuff that I never learned before, that I didn't know, and it [streaming] just took off."